SrasV1, Main, Exploration, bibRecord, 004D30

Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

Identifieur interne : 004D30 ( Main/Exploration ); précédent : 004D29; suivant : 004D31

Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

Auteurs : Shuetsu Fukushi [Japon] ; Tetsuya Mizutani [Japon] ; Masayuki Saijo [Japon] ; Shutoku Matsuyama [Japon] ; Naoko Miyajima [Japon] ; Fumihiro Taguchi [Japon] ; Shigeyuki Itamura [Japon] ; Ichiro Kurane [Japon] ; Shigeru Morikawa [Japon]

Source :

Journal of general virology [ 0022-1317 ] ; 2005.

RBID : Pascal:05-0355487

Descripteurs français

English descriptors

KwdEn :
- Angiotensin-Converting Enzyme Inhibitors (pharmacology), Animals, Carboxypeptidases (antagonists & inhibitors), Carboxypeptidases (metabolism), Chlorocebus aethiops, Dose-Response Relationship, Drug, Hemagglutinins, Viral (metabolism), Hemagglutinins, Viral (physiology), Humans, Membrane Glycoproteins (metabolism), Membrane Glycoproteins (physiology), Microbiology, Peptidyl-Dipeptidase A, Protein, Reassortant Viruses (metabolism), Reassortant Viruses (physiology), Rhabdoviridae Infections (virology), SARS Virus (chemistry), SARS Virus (physiology), Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Vero Cells, Vesicular stomatitis Indiana virus (metabolism), Vesicular stomatitis Indiana virus (physiology), Vesicular stomatitis virus, Viral Envelope Proteins (metabolism), Viral Envelope Proteins (physiology), Virology, Virus Replication.
MESH :
- chemical , antagonists & inhibitors : Carboxypeptidases.
- chemical , metabolism : Carboxypeptidases, Hemagglutinins, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , pharmacology : Angiotensin-Converting Enzyme Inhibitors.
- chemical , physiology : Hemagglutinins, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemistry : SARS Virus.
- metabolism : Reassortant Viruses, Vesicular stomatitis Indiana virus.
- physiology : Reassortant Viruses, SARS Virus, Vesicular stomatitis Indiana virus.
- virology : Rhabdoviridae Infections.
- Animals, Chlorocebus aethiops, Dose-Response Relationship, Drug, Humans, Peptidyl-Dipeptidase A, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Replication.

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSVΔG* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 h after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.

Affiliations:

Le document en format XML

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<author><name sortKey="Miyajima, Naoko" sort="Miyajima, Naoko" uniqKey="Miyajima N" first="Naoko" last="Miyajima">Naoko Miyajima</name>
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<author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
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<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
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<placeName><settlement type="city">Tokyo</settlement>
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<author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name>
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<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
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<term>Animals</term>
<term>Carboxypeptidases (antagonists & inhibitors)</term>
<term>Carboxypeptidases (metabolism)</term>
<term>Chlorocebus aethiops</term>
<term>Dose-Response Relationship, Drug</term>
<term>Hemagglutinins, Viral (metabolism)</term>
<term>Hemagglutinins, Viral (physiology)</term>
<term>Humans</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Microbiology</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Protein</term>
<term>Reassortant Viruses (metabolism)</term>
<term>Reassortant Viruses (physiology)</term>
<term>Rhabdoviridae Infections (virology)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (physiology)</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Vesicular stomatitis Indiana virus (metabolism)</term>
<term>Vesicular stomatitis Indiana virus (physiology)</term>
<term>Vesicular stomatitis virus</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Envelope Proteins (physiology)</term>
<term>Virology</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Carboxypeptidases (antagonistes et inhibiteurs)</term>
<term>Carboxypeptidases (métabolisme)</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Humains</term>
<term>Hémagglutinines virales (métabolisme)</term>
<term>Hémagglutinines virales (physiologie)</term>
<term>Infections à Rhabdoviridae (virologie)</term>
<term>Inhibiteurs de l'enzyme de conversion de l'angiotensine (pharmacologie)</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Relation dose-effet des médicaments</term>
<term>Réplication virale</term>
<term>Virus de la stomatite vésiculeuse de type Indiana (métabolisme)</term>
<term>Virus de la stomatite vésiculeuse de type Indiana (physiologie)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (physiologie)</term>
<term>Virus recombinants (métabolisme)</term>
<term>Virus recombinants (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Carboxypeptidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxypeptidases</term>
<term>Hemagglutinins, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Angiotensin-Converting Enzyme Inhibitors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Hemagglutinins, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Carboxypeptidases</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Reassortant Viruses</term>
<term>Vesicular stomatitis Indiana virus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carboxypeptidases</term>
<term>Glycoprotéines membranaires</term>
<term>Hémagglutinines virales</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus de la stomatite vésiculeuse de type Indiana</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Inhibiteurs de l'enzyme de conversion de l'angiotensine</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Hémagglutinines virales</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus de la stomatite vésiculeuse de type Indiana</term>
<term>Virus du SRAS</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Reassortant Viruses</term>
<term>SARS Virus</term>
<term>Vesicular stomatitis Indiana virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Rhabdoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Rhabdoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Dose-Response Relationship, Drug</term>
<term>Humans</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Relation dose-effet des médicaments</term>
<term>Réplication virale</term>
<term>Virus du SRAS</term>
<term>Virus stomatite vésiculeuse</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Protéine</term>
<term>Microbiologie</term>
<term>Virologie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSVΔG* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 h after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.</div>
</front>
</TEI>
<affiliations><list><country><li>Japon</li>
</country>
<region><li>Région de Kantō</li>
</region>
<settlement><li>Tokyo</li>
</settlement>
</list>
<tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name>
</region>
<name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
<name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name>
<name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name>
<name sortKey="Miyajima, Naoko" sort="Miyajima, Naoko" uniqKey="Miyajima N" first="Naoko" last="Miyajima">Naoko Miyajima</name>
<name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name>
<name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
<name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name>
<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
</country>
</tree>
</affiliations>
</record>

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Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

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